24 strips/box、48 strips/box
Microparticle chemiluminescence immunoassay sandwich principle.
Add sample, analytical buffer, microparticles coated with troponin I ultra antibody, alkaline phosphatase-labeled troponin I ultra antibody into the reaction tube for mixed reaction. After incubation, different sites of troponin I ultra antigen in the sample bind to troponin I ultra antibody on the magnetic beads and troponin I ultra antibody on alkaline phosphatase markers respectively to form a solid-phase antibody antigen enzyme labeled antibody complex. The substances bound to the magnetic beads are adsorbed by the magnetic field, while the unbound enzyme labeled antibodies and other substances are washed away. Then it is mixed with chemiluminescent substrate. The luminescent substrate emits photons under the action of alkaline phosphatase. The amount of photons generated is directly proportional to the concentration of troponin I ultra in the sample. Through the calibration curve of concentration-photon quantity, the concentration of cTnI in the sample can be calculated.
|Microparticles（M）：||0.13mg/ml Microparticles coupled with anti troponin I ultra antibody|
|Reagent 1（R1）：||0.1M Tris buffer|
|Reagent 2（R2）：||0.5μg/ml Alkaline phosphatase labeled anti troponin I ultra antibody|
|Cleaning solution：||0.05% surfactant、0.9% Sodium chloride buffer|
|Substrate：||AMPPD in AMP buffer|
|Calibrator（optional）：||troponin I ultra antigen|
|Control materials（optional）：||troponin I ultra antigen|
1.Components are not interchangeable between batches of reagent strips;
2.See the calibrator bottle label for calibrator concentration;
3.See the control bottle label for the concentration range of controls;
1.Storage: 2℃～8℃, avoid direct sunlight.
2.Validity: unopened products are valid for 12 months under specified conditions.
3.Calibrators and controls after dissolved can be stored for 14 days in 2℃～8℃ dark environment.
Automated CLEIA System of Illumaxbio(lumiflx16、lumiflx16s、lumilite8、lumilite8s).